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課題案例系列之：
抑菌材料
（Antibacterial materials）
案例簡介>>
抑菌材料主要是指基于傳統(tǒng)材料骨架添加各類新型因子而形成的新型復(fù)合材料。
目前主要做過的材料如下：
- 傳統(tǒng)水凝膠的基礎(chǔ)上添加金屬離子（Cu、Ag、As、C、Co等）形成的新型抑菌材料，研究其體外抑菌作用、體內(nèi)安全性、體內(nèi)埋置等；
- 納米纖維復(fù)合材料中添加抗生素、治療藥物等形成的新型復(fù)合材料，研究其體外抑菌作用、細(xì)胞粘附性、腫瘤細(xì)胞殺傷等；
- 新型納米顆粒如Fe3O4偶聯(lián)其他藥物后而形成的新型可吞噬材料，研究其體外抑菌作用、細(xì)胞靶向性、細(xì)胞吞噬情況等；
- 類骨材料載藥后而形成的新型骨替換材料，研究其體外抑菌作用，膠原細(xì)胞附著、體內(nèi)修復(fù)作用等；
技術(shù)路線>>
結(jié)果展示>> Fig1
A B C D
E
A. After adding new factors to the material, the viscosity and adhesion become stronger;
B. The new material can significantly heat up due to its absorption of specific wavelength light after adding special factors.
C. The drug carrier material can stably release the drug carried;
D. Analysis of the main composition of materials;
E. New materials undergo spatial changes due to the addition of specific factors.
技術(shù)路線>>
結(jié)果展示>> Fig2
A B C D E
F
|
OD405 |
調(diào)零 |
均值 |
|
溶血率 |
||
|
空白材料 |
1.315 |
1.276 |
1.326 |
1.306 |
|
24.200 % |
|
新材料A |
0.573 |
0.601 |
0.599 |
0.591 |
|
7.269 % |
|
陰性 |
0.278 |
0.285 |
0.290 |
0.284 |
|
|
|
陽性 |
3.977 |
4.437 |
5.098 |
4.504 |
|
|
?A. The new material has stronger antibacterial effect due to the addition of new factors, and the antibacterial zone is significantly increased;
B. The new material has stronger antibacterial effects due to the addition of new factors, making it almost impossible for bacteria to grow on it, resulting in almost no living bacteria in the eluate;
C. The drugs released by antibacterial materials have a great destructive effect on the biofilm and have a significant antibacterial effect;
D. The drugs released from antibacterial materials have a significant inhibitory effect on bacteria, and dead bacteria can be stained red by PI due to enhanced membrane permeability;
E. The drugs released from antibacterial materials have a significant inhibitory effect on bacteria, causing bacterial shrinkage and cell membrane dissolution on the materials, leading to cell apoptosis;
F. The release of new materials has no impact on hemolysis;
???技術(shù)路線>>
結(jié)果展示>> Fig3
A B C
?
A. The CCK proliferation curve verifies that cells undergo apoptosis when attacked by bacteria, and the new material can release more antibacterial molecules to rescue this process;
B. The flow cytometry apoptosis experiment verifies that cells undergo a large amount of apoptosis when attacked by bacteria, and the new material can release more antibacterial molecules to rescue this process;
C. The mRNA sequencing results reveal specific response signaling pathways. Comparing the model group with the blank group, mRNA sequencing screened for significantly up-regulated or down-regulated factors, thus suggesting the possible pathways through which antibacterial materials may achieve cell protection mechanisms
技術(shù)路線>>
結(jié)果展示>> Fig4
A B C D
A. RT-qPCR detection explores the specific pathways of action of antibacterial materials at the mRNA level to protect cells from bacterial invasion;
B. WB detection explores the specific pathways of action of antibacterial materials at the protein level to protect cells from bacterial invasion;
C. Biochemical Elisa verifies that cells are protected by new materials to avoid excessive inflammatory reactions.
技術(shù)路線>>
結(jié)果展示>> Fig5
?A B
C D E
A. CCK8 proliferation level verification: The new material specifically protects cells from bacterial invasion through which pathway;
B. Flow cytometry apoptosis level verification: The specific pathway through which the new material protects cells from bacterial invasion;
C. Cell ferroptosis can be reversed by overexpression of the target protein, and the blocker of the target protein can block this process, resulting in the recovery of ferroptosis related protein factors. This experiment detected the expression of related proteins in cells through Elisa's method;
D. The protein level validation of the novel material specifically protects cells from bacterial invasion through which pathway;
E. Elisa Assay detects protein factor levels and verifies which pathway the new material specifically protects cells from bacterial invasion through;
技術(shù)路線>>
結(jié)果展示>> Fig6
A B C D E
A. In vivo experiments shows that new materials can release more antibacterial molecules to inhibit bacterial growth, thereby helping the matrix avoid inflammation caused by bacteria;
B. The HE results show that the new material helps the matrix to prevent inflammation caused by bacteria;
C. The WB results verify that the new material can release more antibacterial molecules to inhibit bacterial growth, thereby helping the matrix avoid inflammation caused by bacteria;
D. RT-qPCR detection verifies that the new material can release more antibacterial molecules to inhibit bacterial growth, thereby helping the matrix avoid inflammation caused by bacteria;
E. Elisa Assay confirms that the new material can release more antibacterial molecules to inhibit bacterial growth, thereby helping the substrate avoid inflammation caused by bacteria.